Journal: PLoS ONE
Article Title: In Vitro neurotoxicity and myotoxicity of Malaysian Naja sumatrana and Naja kaouthia venoms: Neutralization by monovalent and Neuro Polyvalent Antivenoms from Thailand
doi: 10.1371/journal.pone.0274488
Figure Lengend Snippet: (i) N . sumatrana and: (ii) N . kaouthia venoms (10 μg) were separated in a 10% gel, blotted into a PVDF membrane and visualized using a chemiluminescence substrate. Panel (A) SDS- PAGE profile of venoms. Panel (B-D) are blot membrane that were incubated with Thai Neuro Polyvalent Antivenom, Thai Monocled Cobra Antivenom, and Thai King Cobra Antivenom, respectively. M is lane for protein molecular weight marker.
Article Snippet: The 2x Laemli SDS-PAGE buffer (Bio- Rad, Hercules, USA) was used as non-reducing sample buffer and the reducing sample buffer was prepared by mixing 50 μL of β-mercaptoethanol (Bio- Rad, Hercules, USA) per 950 μL of 2x Laemli SDS-PAGE sample buffer (Bio- Rad, Hercules, USA).
Techniques: Membrane, SDS Page, Incubation, Combined Bisulfite Restriction Analysis Assay, Molecular Weight, Marker